PI(4)P PolyPIPosomes

Catalog No.:  Y-P004

Description:  PolyPIPosomes are polymerized liposomes that show markedly increased stability (up to 6 months) vs. conventional liposomes. A biotin tag is incorporated for easy detection of PIPn-binding proteins with streptavidin reagents.
- Uniform size (~200 nm)
- Ready-to-use suspensions, no liposome preparation
PolyPIPosomes have demonstrated utility in a number of assay formats:
  • -Protein pull-down
  • -Surface Plasmon Resonance
  • -Liposome overlay assay

PI(4)P PolyPIPosomes are provided as a 1mM total lipid solution containing 5% PI(4)P. Each order of 250 μL or 1 mL of PolyPIPosomes includes 100 μL of Control PolyPIPosomes (Y-0000).

Featured in Publications:

1) Ferguson, C. G., R. D. James, et al. (2005). "Phosphoinositide-Containing Polymerized Liposomes: Stable Membrane-Mimetic Vesicles for Protein-Lipid Binding Analysis." Bioconjug Chem 16(6): 1475-1483.
2) Elkin, S. K., D. Ivanov, et al. (2005). "A PHD finger motif in the C-terminus of RAG2 modulates recombination activity." J Biol Chem 280(31): 28701-10.
3) Mustafa, A. K., D. B. van Rossum, et al. (2009). "Glutamatergic regulation of serine racemase via reversal of PIP2 inhibition." Proc Natl Acad Sci U S A 106(8): 2921-6.
4) Zemskov, E. A., I. Mikhailenko, et al. (2011). "Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes." PLoS One 6(4): e19414.
5) Weber, S. S., C. Ragaz, et al. (2006). "Legionella pneumophila exploits PI(4)P to anchor secreted effector proteins to the replicative vacuole." PLoS Pathog 2(5): e46.
Xu, Q., Y. Zhang, et al. (2016). "Phosphatidylinositol phosphate kinase PIPKI[gamma] and phosphatase INPP5E coordinate initiation of ciliogenesis." Nat Commun 7. doi:10.1038/ncomms10777

Being bio-reactive blog: "Lipid-Ligand interaction tools: lipids are not scary intermediates anymore"

Options/Sizes Pricing
Y-P004-100ul $ 217.00
Y-P004-250ul $ 517.00
Y-P004-1ml $ 1,935.00
Additional Information:
Tech Data Sheet - TDS_Y-P00xx_ Rev8

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assay and reagents for drug discovery in lipid signaling pathways