Malachite Green Assay Kit

Catalog No.:  K-1500

Description:  Echelon’s Malachite Green Phosphatase Assay kits are designed for the in vitro measurement of lipid and protein phosphatase activity. The liberated phosphate forms a colored complex with molybdate/malachite green, which is quantified by reading the absorbance at 620 nm. The Echelon assay uses water-soluble lipid substrates so there is no need to make phospholipid vesicles. The assay is fast, convenient and offers a flexible non-radioactive alternative for measuring specific phosphatase activities. The Malachite Green phosphatase assays can measure as little as 50 pmoles of free phosphate and has a high level of accuracy in the range of 200 to 2,000 pmol of phosphate per assay point.


The kit includes enough reagents for 192 samples, standards, and controls.

Storage/ Stability:

> 6 months at 4° C

The malachite green assay can be purchased with lipid substrates

Choice of substrates: 2 substrates K-1520, 3 substrates K-1530, 4 Substrates K-1540

Featured in Publication:

Options/Sizes Pricing
K-1500-1ea $ 77.00
Additional Information:
Tech Data Sheet - TDS_K-1500_ Rev 5

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assay and reagents for drug discovery in lipid signaling pathways