Fluorescein conjugated Anti-PI(4,5)P2 IgM
Catalog No.: Z-G045
Description: Purified Anti-PtdIns(4,5)P2 monoclonal antibody conjugated with fluorescein.
Applications: ELISA, Immunofluorescence
Concentration: 0.5 - 1 mg/mL depending on lot number. Please see lot specific TDS.
Product Background: Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to play a central role in a variety of cellular functions. Amongst its many functions, PIP2 is a substrate for Phospholipase C-coupled G-protein pathways involved in intracellular calcium release in a number of tissues. It is also a substrate for class I phosphoinositide 3-kinase (PI3-K) forming PIP3.
StorageProtect from light. 2-8 °C for up to 30 days, -20 °C for greater than 30 days. Do not freeze thaw multiple times
Featured in Publications1) Antony, P., K. Hoek, et al. (2007). "Micro-scale flow cytometry-based and biochemical analysis of lipid signaling in primary B cell subpopulations." Biol Proced Online 9: 73-83.
2) Heuvingh, J., M. Franco, et al. (2007). "ARF1-mediated actin polymerization produces movement of artificial vesicles." Proc Natl Acad Sci U S A 104(43): 16928-33.
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Product keywords: PIP2, PI(4,5)P2, PtdIns(4,5)P2, IgM, anti-PIP2
Tech Data Sheet - TDS Z-G045 Rev 5x
Protocol - Protocol for immunofluorescence in chamber slides
Protocol - Protocol for frozen tissue sections