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PI(3,4,5)P3 PIP Beads™


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Catalog No.:  P-B345a

Description:  PI(3,4,5)P3 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(3,4,5)P3 per mL of beads, enough for several protein binding experiment. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.

Storage

  • 4 °C - do not freeze

Applications

  • PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.

Featured in Publications

1) Hiromura, M., F. Okada, et al. (2004). "Inhibition of Akt kinase activity by a peptide spanning the betaA strand of the proto-oncogene TCL1." J Biol Chem 279(51): 53407-18.
2) Craig, H. E., J. Coadwell, et al. (2010). "ARAP3 binding to phosphatidylinositol-(3,4,5)-trisphosphate depends on N-terminal tandem PH domains and adjacent sequences." Cellular Signalling 22(2): 257-264.
3) Takahashi, K. and K. Suzuki (2010). "WAVE2 targeting to phosphatidylinositol 3,4,5-triphosphate mediated by insulin receptor substrate p53 through a complex with WAVE2." Cellular Signalling 22(11): 1708-1716.
4) Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039. http://www.bio-protocol.org/e1039

Being bio-reactive blog: "Lipid-Ligand interaction tools: lipids are not scary intermediates anymore"


Options/Sizes Pricing
P-B345a-100ul $ 99.00
P-B345a-1ml $ 515.00
Additional Information:
Tech Data Sheet - TDS_P-Bxxx_ Rev 7

Protocol - Protocol


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assay and reagents for drug discovery in lipid signaling pathways