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PI(4)P PIP Beads™


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Catalog No.:  P-B004a

Description:  PI(4)P PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(4)P per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.

Storage

  • 4 °C - do not freeze

Applications

  • PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.

Featured in Publication

1) Casamayor, A. and M. Snyder (2003). "Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function." Mol Cell Biol 23(8): 2762-77.
2) Hu, Y., Z. Liu, et al. (2005). "Phosphoinositol lipids bind to phosphatidylinositol 3 (PI3)-kinase enhancer GTPase and mediate its stimulatory effect on PI3-kinase and Akt signalings." Proc Natl Acad Sci U S A 102(46): 16853-8.
3) Brombacher, E., S. Urwyler, et al. (2009). "Rab1 guanine nucleotide exchange factor SidM is a major phosphatidylinositol 4-phosphate-binding effector protein of Legionella pneumophila." J Biol Chem 284(8): 4846-56.

Being bio-reactive blog: "Lipid-Ligand interaction tools: lipids are not scary intermediates anymore"


Options/Sizes Pricing
P-B004a-0.1ml $ 99.00
P-B004a-1ml $ 515.00
Additional Information:
Tech Data Sheet - TDS_P-Bxxx_ Rev 7

Protocol - Protocol


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assay and reagents for drug discovery in lipid signaling pathways