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PKC delta, active


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Catalog No.:  E-K037

Description:  Recombinant full-length human PKC delta containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells.
The gene accession number is NM_006254.
This material is sold for research purposes only.
Specific Activity
302 nmol phosphate incorporated into CREBtide substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Scientific Background

  • Protein kinase C delta (PKC delta) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kd protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Phosphatidylinositol is the most potent activator of PKC delta. Apparent kinetic constants for synthetic oligopeptides (MBP4-14, EGFR peptide and epsilon-peptide) are quite different between PKC delta and other PKCs. Northern blot analysis indicated that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells (2). PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters. PKC delta is partially associated with the insoluble fraction in cells even in the absence of phorbol 12-myristate 13-acetate (PMA). Upon PMA stimulation, both it translocate to the insoluble fraction of cell homogenates (3). Overexpression of PKC-delta induces significant changes

Storage

  • Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.

References

  • 1. Leibersperger H, Gschwendt M, Gernold M, Marks F. Immunological demonstration of a calcium-unresponsive protein kinase C of the delta-type in different species and murine tissues. Predominance in epidermis. J Biol Chem. 1991 Aug 5;266(22):14778-84.2. Mischak H, Bodenteich A, Kolch W, Goodnight J, Hofer F, Mushinski JF. Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein. Biochemistry. 1991 Aug 13;30(32):7925-31. 3. Mischak H, Goodnight JA, Kolch W, Martiny-Baron G, Schaechtle C, Kazanietz MG, Blumberg PM, Pierce JH, Mushinski JF. Overexpression of protein kinase C-delta and -epsilon in NIH 3T3 cells induces opposite effects on growth, morphology, anchorage dependence, and tumorigenicity. J Biol Chem. 1993 Mar 25;268(9):6090-6.


Options/Sizes Pricing
E-K037-5ug $ 158.00
E-K037-20ug $ 474.00
Additional Information:
Tech Data Sheet - E-K037


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