Autotaxin Activity Assay

Catalog No.:  K-4100

Description:  Echelon's Autotaxin Activity Assay gives a quantitative measurement of autotaxin (ATX) activity in biological samples by measuring changes in fluorescence over time.

Sample Volume: 400 μL / sample (50 μL per well, 8 wells per sample)
Sample Number: 10 samples (in duplicate) / 96 well assay
Sample Type: Currently this kit has been tested for the quantification of autotaxin activity in serum, plasma, ovarian ascites, and cell culture media.

Product Background: The assay developed by Echelon Biosciences Inc. uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence. Once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence.
This assay gives a quantitative measurement of autotaxin activity in biological samples by measuring changes in fluorescence over time. Because the assay is not end point based, it has the advantage of being less susceptible to error arising from mistiming the addition of components to different sample wells. The final readout is in quantitative units that correct for inconsistencies between different users equipment.

Featured in Publications:

1) Shelton, E. L., C. L. Galindo, et al. (2013). "Autotaxin Signaling Governs Phenotypic Heterogeneity in Visceral and Parietal Mesothelia." PLoS ONE 8(7): e69712.
2) Gaetano, C. G., N. Samadi, et al. (2009). "Inhibition of autotaxin production or activity blocks lysophosphatidylcholine-induced migration of human breast cancer and melanoma cells." Molecular Carcinogenesis 48(9): 801.
3) Braeuer, R. R., M. Zigler, et al. (2012). "Galectin-3 Contributes to Melanoma Growth and Metastasis via Regulation of NFAT1 and Autotaxin." Cancer Research 72(22): 5757-5766.

Related Products:
Autotaxin Inhibitor Screening Kits: 96-well (K-4200) and 384-well (K-4200HTS)
Autotaxin Substrate: FS-3 (L-2000)
Autotaxin Inhibitors: BrP-LPA (L-7416), HA130 (B-0701), PF8380 (B-0702), and Thio-ccPA (L-7118 )
Autotaxin Enzyme, active (E-4000)

Notice to Purchaser:Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patent 7,989,663. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email

Product Keywords: ATX, LPA, FS-3, LPC, lysophospholipase D

Options/Sizes Pricing
K-4100-1ea $ 487.00
Additional Information:
Tech Data Sheet - TDS _K-4100_ rev8x

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assay and reagents for drug discovery in lipid signaling pathways