PI3-Kinase Activity ELISA: Pico
Catalog No.: K-1000s
Description: Echelon's PI3-Kinase Activity ELISA: Pico is a competitive (96-well) ELISA assay kit that measures Class I Phosphoinositide 3-kinases (PI3-K) activity directly through its reaction product, PtdIns(3,4,5)P3 (PIP3). This assay can be used for in vitro determination of PI3-K activity in cancer tissues and cell lines or compound screening for drug discovery
Assay sensitivity: This assay can measure as low as 0.22 pmol PIP3 and detect activity from as little as 31 ng/mL PI3-K enzyme
Assay Incubation time: 2.5 hr
Sample Type: Purified and/ or Immunoprecipitated (IP) PI3-K Enzyme (see IP protocol at the end of the K-1000s TDS)
Product Background: PI3-K reactions are run with the Class I PI3-K physiological substrate PI(4,5)P2 (PIP2). The enzyme reactions, PIP3 standards, and controls are then mixed and incubated with PIP3 binding protein that is highly specific and sensitive to PIP3. This mixture is then transferred to a PIP3-coated microplate for competitive binding. Afterwards, a peroxidase-linked secondary detector and colorimetric detection is used to detect the amount of PIP3 produced by PI3-K through comparing the enzyme reactions with a PIP3 standard curve.
Featured in Publications:1. A. E. Awad, V. Kandalam, S. Chakrabarti, X. Wang, J. M. Penninger, S. T. Davidge, G. Y. Oudit and Z. Kassiri. (2010) "Tumor necrosis factor induces matrix metalloproteinases in cardiomyocytes and cardiofibroblasts differentially via superoxide production in a PI3K?-dependent manner" Am J Physiol Cell Physiol 298(3), C679-C692
2. B. Venkatesan, S. D. Prabhu, K. Venkatachalam, S. Mummidi, A. J. Valente, R. A. Clark, P. Delafontaine and B. Chandrasekar. (2010) "WNT1-inducible signaling pathway protein-1 activates diverse cell survival pathways and blocks doxorubicin-induced cardiomyocyte death" Cell Signal 22(5), 809-20
3. J. J. Wallin, J. Guan, K. A. et al. (2012) "Active PI3K Pathway Causes an Invasive Phenotype Which Can Be Reversed or Promoted by Blocking the Pathway at Divergent Nodes" PLoS One 7(5), e36402
4. C. M. Furtado, M. C. Marcondes, R. S. Carvalho, M. Sola-Penna and P. Zancan. (2015) "Phosphatidylinositol-3-kinase as a putative target for anticancer action of clotrimazole" The International Journal of Biochemistry & Cell Biology 62, 132-141
5. Poopal, A. C., L. M. Schroeder, et al. (2016). Increased expression of the PI3K catalytic subunit p110? underlies elevated S6 phosphorylation and protein synthesis in an individual with autism from a multiplex family. 7: 1-12. doi: 10.1186/s13229-015-0066-4
Class I PI3K Activity Assays: Echelon has multiple formats available. Please see the PI3-K Assay Comparison Chart for more information.
Class III PI3K Activity Assay (K-3000)
Lipid Mass ELISA's: PIP3 Mass ELISA (K-2500s) and PI(4,5)P2 Mass ELISA (K-4500)
Human PI3K Enzymes: alpha (p110α/p85α), beta (p110β/p85α), PI3Kα E545K – Active Mutant (p110α/p85α)
Mouse PI3K Enzyme: PI3K (p110α/p85α)
Notice to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. This kit and all non-radioactive, competitive assays for determining phosphoinositide-3-kinase (PI3-K) activity are covered by Echelon Biosciences Inc. U.S. Patent 7,067,269. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email firstname.lastname@example.org.
Product Keywords: PI3-Kinase, PI3K, Phosphatidylinositol 3-kinases, PI 3-kinases, PI3Ks, K-1000
Bulk Discounts: Bulk discounts are available, please email email@example.com for details
Tech Data Sheet - TDS K-1000s PI3-K ELISA Pico Rev 6x