Hyaluronic Acid Sandwich ELISA

Catalog No.:  K-4800

Description:  The Hyaluronic Acid Sandwich ELISA, a 96-well assay, offers a simple, effective method for determining Hyaluronic Acid (HA) levels in human and animal biological fluids or cell supernatants.

Assay Range: 12.5-3200 ng/ml
Sensitivity: 12.5 ng/mL
Sample Volume: 25 μL

Echelon’s Hyaluronic Acid Sandwich ELISA is a quantitative immunoassay designed for in vitro measurement of HA levels in cell culture supernatant, or human/animal biological fluids. The concentration of HA in the sample is determined using a standard curve of known amounts of HA. Echelon’s HA Sandwich ELISA was validated with human sera samples. Only a small amount of sample (25 μL) is needed for running duplicates measurements. Echelon’s HA Sandwich ELISA provides a robust and simple method for researchers to measure HA in biological samples.


Part 1 at -20 °C and part 2 at 4 °C.

Featured in Publications:

1) D. Bruegger, F. Brettner, I. Rossberg, C. Nussbaum, C. Kowalski, K. Januszewska, B. F. Becker and D. Chappell. (2015) "Acute Degradation of the Endothelial Glycocalyx in Infants Undergoing Cardiac Surgical Procedures" The Annals of Thoracic Surgery 99(3), 926–931 doi: 10.1016/j.athoracsur.2014.10.013
2) J. Qin, J. Kilkus and G. Dawson. (2015) "The hyaluronic acid inhibitor 4-methylumbelliforone is an NSMase2 activator-A novel antitumor mechanism of MU" Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1861(2), 78-90 doi:10.1016/j.bbalip.2015.11.001

Being Bio-reactive blog: "How to select Hyaluronic Acid (HA) quantitative tests?"

Related Products:

Collagen IV ELISA Kit (K-1700)
Hyaluronic Acid ELISA (K-1200)
Hyaluronidase Activity ELISA (K-6000)

Product Keywords: Hyaluronan, Hyaluronate, Hyaluronic Acid, Liver Cirrhosis, Rheumatoid Arthritis, Osteoarthritis, Liver Fibrosis

Options/Sizes Pricing
K-4800-1kit $ 568.00
Additional Information:
FAQ - FAQ_HA Assays Comparison Chart_Rv1

Tech Data Sheet - TDS_K-4800_Rev8

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assay and reagents for drug discovery in lipid signaling pathways