Products

ATX-Red AR-2


Zoom
 
Catalog No.:  L-2010
MW: 3331.81
Purity: >90%

Description:  ATX-Red AR-2 is an in vivo imaging probe for visualizing and measuring ATX activity. It is an analog of the autotaxin substrate lysophosphatidylcholine (LPC) and contains a near-infrared fluor (Licor IRDye® 800CW) and quencher (Licor IRDye® QC-1). The design is modeled after FS-3, a fluorogenic ATX substrate that is predictive of ATX activity. In the parent compound, fluorescence is quenched through intramolecular energy transfer, but when autotaxin cleaves ATX-Red AR-2, fluorescence increases. This activation mechanism is specific to autotaxin in vitro and in vivo.

Autotaxin which has lysophospholipase D (lysoPLD) activity, cleaves choline from lysophosphatidylcholine forming lysophosphatidic acid (LPA), a potent mitogen that as been implicated in the pathophysiology of ovarian cancer. LysoPLD/ATX has been demonstrated to increase cell motility, neovascularization, proliferation, and aggressiveness of tumors and is upregulated in numerous cancer lineages (non-small cell lung , glioma, mammary carcinoma, renal cell carcinoma, hepatocellular carcinoma). In addition, dysregulation of the ATX/LPA pathway is central to the pathophysiology of idiopathic pulmonary fibrosis, rheumatoid arthritis, and other inflammatory diseases. Modulation of ATX activity through small-molecules is a currently underexplored target, but one with high potential for novel cancer and anti-inflammatory therapeutics.

Storage

-20 °C, protected from light

Excitation/Emission

775/800 nm

Featured in Publications:

Madan, D., C.G. Ferguson, et al. (2013) "Non-Invasive Imaging of Tumors by Monitoring Autotaxin Activity Using an Enzyme-Activated Near-Infrared Fluorogenic Substrate" PLoS One 8(11): e79065

Related Products:

Autotaxin Inhibitor Screening Kits: 96-well (K-4200) and 384-well (K-4200HTS)
Autotaxin Activity Assay (K-4100)
Autotaxin ELISA (K-5600)
Autotaxin Inhibitors: BrP-LPA(L-7416), HA130 (B-0701), S32826 (L-3282), and Thio-ccPA (L-7118)
Autotaxin Enzyme, active (E-4000)

Notice to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound AR-2 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patents 7,485,259 & 7,989,663. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email busdev@echelon-inc.com

Bulk discounts available, please email echelon@echelon-inc.com for information.


Options/Sizes Pricing
L-2010-10ug $ 78.00
L-2010-50ug $ 380.00
L-2010-100ug $ 600.00
Additional Information:
Tech Data Sheet - L-2010

FAQ - AACR 2013 poster


Add a specific quantity to your cart    *  


Back to the webstore startpage      Back to the product overview      Your Shopping Cart      Terms of Service


assay and reagents for drug discovery in lipid signaling pathways