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Autotaxin Sandwich ELISA


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Catalog No.:  K-5600

Description:  Description: Echelon's ATX Sandwich ELISA is a quantitative immunoassay designed for in vitro measurement of ATX levels in cell culture supernatant or human and mouse biological fluids.
Sample Volume: 5μL
Sample Number: 40 samples (in duplicate) / 96-well assay
Sample Type: Currently this kit has been tested for the quantification of ATX levels in cell culture supernatant, sera, plasma, urine and ovarian ascites.



Product Background: Autotaxin, also known as ATX, ENPP2, lysophospholipase D (lysoPLD), phosphodiesterase 1α and plasma cell glycoprotein-1, is a secreted glycoprotein that is widely expressed with high levels in the serum. Via its lysoPLD activity, autotaxin hydrolyzes lysophosphatidylcholine (LPC) to generate the phospholipid growth factor lysophosphatidic acid (LPA). The enzyme’s same activity hydrolyzes sphingosylphosphorylcholine (SPC) to form sphinosine-1-phosphate (S1P). Autotaxin was first isolated as the autocrine motility factor secreted from melanoma cells. At that time, the enzyme’s lysoPLD activity had not been identified, so it was classified by homology to the ecto-nucleotide pyrophosphatase/phosphodiesterase (NPP) family of enzymes whose members hydrolyze phosphodiester bonds in various nucleotides and nucleotide derivatives. It was initially unclear how nucleotide hydrolysis could lead to the stimulation of cell motility. This mystery was solved when autotaxin was discovered to be identical to serum lysoPLD. Since then, the cancer-related activities of autotaxin, at least in cultured cells, have been attributed to the enzyme’s lysoPLD activity. In addition to cancer, autotaxin has been implicated in a number of diseases including obesity, arthritis, multiple sclerosis, Alzheimer’s disease and neuropathic pain.
Echelon’s ATX Sandwich ELISA provides a robust and simple method for researchers to measure ATX in biological samples. The concentration of ATX in the sample is determined using a standard curve of known amounts of ATX. The assay was validated with sera, plasma, urine and ovarian ascites.

Related Products:
Autotaxin Activity Assay (K-4100)
Autotaxin Inhibitor Screening Kits: 96-well (K-4200) and 384-well (K-4200HTS)
Autotaxin Substrate: FS-3 (L-2000)
Autotaxin Inhibitors: HA-130 (B-0701), BrP-LPA (L-7416) and Thio-ccPA (L-7118 )
Autotaxin Enzyme, active (E-4000)

Product Keywords: Autotaxin, ATX, LPA, LPC, lysophospholipase D


Options/Sizes Pricing
K-5600-1kit $ 525.00
Additional Information:
Tech Data Sheet - TDS_K-5600_Rev3


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assay and reagents for drug discovery in lipid signaling pathways