PI(5)P 4-Kinase (PIP4KII) Activity Assay

Catalog No.: K-5400

Description: Echelon’s PI(5)P 4-Kinase Activity Assay is an ATP depletion assay which quantifies the remaining ATP levels in solution following the kinase reaction. The assay can be used to quantify PI(5)P 4-Kinase in vitro or in cell lysate.

Type II PI(5)P 4-Kinase (PIP4KII) catalyzes the phosphorylation of phosphatidylinositol-5-phosphate [PI(5)P] to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. PIP4KII has been shown to play a role in certain types of breast and colon cancer in addition to being important in several physiological processes such as insulin signaling, stress response, and embryonic development in several model organisms. It has also been implicated as an important regulator of nuclear PI(5)P levels.
The assay reaction is prepared by the addition of PIP4KII (enzyme), PI(5)P (substrate) in reaction buffer and initiated by the addition of ATP. The reaction is then stopped by adding the ATP detector after the chosen reaction time. The luminescent signal is inversely correlated with the kinase activity. Echelon’s PI(5)P 4-Kinase Activity Assay is tested using SignalChem’s PIP4KIIA (P76-10AG) kinase.

Storage

-20 °C

Related Products:

Substrates: P-5016, P-5008
Related Enzymes: PI3Kα (E-2000), PTEN (E-3000), SHIP2 (E-1000)
Related Assays: PI(4)P 5-Kinase (PIP4KI) Activity Assay (K-5700), PI3-Kinase Activity ELISA (K-1000s), PTEN Activity ELISA (K-4700)

Options/Sizes	Pricing
K-5400-1kit	$ 613.00

Additional Information:
Tech Data Sheet - TDS_K-5400_Rev 3

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assay and reagents for drug discovery in lipid signaling pathways