Sphingosine-1-Phosphate Lyase Overexpression Lysate, active
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Catalog No.: E-5000L Description: Sphingsoine-1-phosphate lyase (SPL) (E.C.4.1.2.27) works as a key regulator of the final step of the sphingolipid degradative pathway by irreversibly breaking down the sphingosine-1-phosphate (S1P) to ethanolamine phosphate and hexadecenal. S1P signaling through its five receptors involves in many biological events such as proliferation, inflammation and apoptosis in cancer and other degenerative diseases. |
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HEK293 cell lysate with overexpressed SPL-GFP (green fluorescent protein). Storage-80°C. The enzyme is stable for 6 months from the date of receipt. Avoid repeat freeze thaw cycles.References1. Billich, A., et al. (2013). "Partial deficiency of sphingosine-1-phosphate lyase confers protection in experimental autoimmune encephalomyelitis." PLoS One 8(3): e59630.2. Engel, N., et al. (2012). "Metabolic profiling reveals sphingosine-1-phosphate kinase 2 and lyase as key targets of (phyto-) estrogen action in the breast cancer cell line MCF-7 and not in MCF-12A." PLoS One 7(10): e47833. 3. Bedia, C., et al. (2009). "Synthesis of a fluorogenic analogue of sphingosine-1-phosphate and its use to determine sphingosine-1-phosphate lyase activity." Chembiochem 10(5): 820-2. 4. Reiss,U., et al. (2004) "Sphingosine-phosphate Lyase Enhances Stress-induced Ceramide Generation and Apoptosis." J. Biol. Chem. 279(2): 1281-1290. Related products Sphingosine-1-Phosphate Lyase Fluorogenic Substrate (S-200U) 2-Acetyl-5-tetrahydroxybutyl imidazole (THI) (B-0031) Sphingolipids Sphingosine 1-Phosphate ELISA kit (K-1900) |
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Additional Information:
Tech Data Sheet - TDS_E-5000L_Rev1 |
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