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PI(4)P Mass ELISA Kit


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Catalog No.:  K-4000E

Description:  Echelon's PI(4)P Mass ELISA kit is designed to detect and quantify PI(4)P by means of a competitive ELISA format, eliminating the need for radioactivity and thin layer chromatography. The PI(4)P Mass ELISA directly detects PI(4)P over all other phosphonositides at relevant biological levels.

Assay Range: 50-0.7 pmol PI(4)P
Sample Type: Dried down lipid extracted from cells
Sample Volume: 60 μL/sample, single point

Product Background:The PI(4)P Mass assay is a competitive ELISA in which the signal is inversely proportional to the amount of PI(4)P measured. The PI(4)P extractions are added to the colored mixing plate with PI(4)P Grip. This mixture is then transferred to the PI(4)P Detection Plate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect the amount of PI(4)P Grip binding to the plate. The assay is sensitive to 0.7 pmol PI(4)P.

Storage

The kit comes in two parts with different storage requirements. Upon receipt store Kit Part 1 at 4°C and Kit Part 2 at -20°C. Store prepared reagents as indicated in the protocol.

Featured in References

1) S. Choi, et al. "Agonist-stimulated phosphatidylinositol-3,4,5-trisphosphate generation by scaffolded phosphoinositide kinases" Nature Cell Biology (2016) 18(12), 1324-35, doi:10.1038/ncb3441

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Options/Sizes Pricing
K-4000E-1kit $ 567.00
Additional Information:
Tech Data Sheet - TDS_K-4000E_rev7

FAQ - FAQ PIP Mass rev 6


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assay and reagents for drug discovery in lipid signaling pathways