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Collagen Hybridizing Peptide - Fluorescein Conjugate


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Catalog No.:  FLUCHP
MW: 2952
Purity: 95%

Description:  Formerly cat# C-660F

b>The Collagen Hybridizing Peptides are products of 3Helix Inc.

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo [1,2,3]. By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.
CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging [3]. CHP can measure and localize mechanical injury to collagenous tissue at the molecular level [4]. It also enables assessment of collagen denaturation in decellularized extracellular matrix [5]. In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot [6]. B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options, such as non-green fluorescence and HRP-enzyme methods, to avoid background and enhance signal.

CHP conjugates are labeled with either 5-FAM (F-CHP, Catalog #: FLUCHP) for fluorescence detection or biotin (B-CHP, Catalog #: BIOCHP) for avidin/streptavidin-mediated detection. For more examples of CHP’s applications, please visit http://www.3helix.com/applications/.

Applications: immunofluorescence, immunohistochemistry, SDS-PAGE (in-gel Western blot)

Shipping Conditions

Express shipping as powder at ambient temperature. Store at -20 °C upon arrival until use.

Storage

-20 °C as powder; 4 °C after reconstitution in water, no need to aliquot and freeze.

References

1) “Targeting and mimicking collagens via triple helical peptide assemblies” Curr Opin Chem Biol, 2013, 17, 968-975.
2) “Targeting collagen strand by photo-triggered triple-helix hybridization” Proceedings of the National Academy of Sciences of the United States of America, 2012, 109, 14767-14772. DOI: 10.1073/pnas.1209721109.
3) "In situ imaging of tissue remodeling with collagen hybridizing peptides." ACS Nano, 2017, 11, 9825. DOI: 10.1021/acsnano.7b03150
4) "Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides." Nat. Commun., 2017, 8, 14913, DOI: 10.1038/ncomms14913.
5) 5. Molecular assessment of collagen denaturation in decellularized tissues using the collagen hybridizing peptide. Acta Biomater., 2017. 53, 268-278, DOI: 10.1016/j.actbio.2017.01.079
6) “Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides” Bioconjugate Chemistry, 2013, 24, 9-16. DOI: 10.1021/bc3005842.

Features
-More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
-High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
-Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
-Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
-A non-antibody approach with no species restrictions against any co-staining antibody
Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
-Stable in solution under 4 °C, eliminating the need to aliquot for storage

Related Products

Collagen IV ELISA Kit II (K-1700s)


Options/Sizes Pricing
FLUCHP-60ug $ 198.00
FLUCHP-300ug $ 550.00
Additional Information:
Tech Data Sheet - TDS_FLUCHP_rev1


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