PI4-Kinase II alpha (PI4KIIa), active

Catalog No.:  P21-10G

Description:  Description: Full-length recombinant human PI4KIIA was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Enzyme obtained from SignalChem.

Molecular weight: ~79 kDa

Sequence: Full length

Species: Human

Tag: GST tag

Purity: >90 % (see TDS for lot specific information)

Specific Activity: >30 nmol/min/mg (see TDS for lot specific information)
Expression System: Sf9 insect cells using baculovirus

Genebank Number: NM_018425

Applications: PI 4-Kinase assay, western blot.

Formulation: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Storage and stability: Store product at -70ÂșC. Stable up to one year.

Scientific Background: PI4KIIA or phosphatidylinositol 4-kinase type 2 alpha is involved in the formation of phosphatidylinositolpolyphosphates (PtdInsPs) which are centrally involved in many biologic processes ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KIIA phosphorylates PtdIns at the D-4 position which is an essential step in the biosynthesis of PtdInsPs. PI4KIIA is a novel regulator of tumor growth by its action on angiogenesis and HIF-1alpha regulation. PI4KIIA generates PtdIns4P-rich domains within the Golgi which regulate targeting of clathrin adaptor AP-1 complexes. PI4KIIA is also required for Wnt to regulate LRP6 phosphorylation.

Related Products:

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PI 4-Kinase Activity ELISA (K-4000K)

PIk-93; PI 4-kinase Inhibitor (B-0306)

PI4KIIb (P32-10G)

PI4KIIIb (P-22-10G)

Options/Sizes Pricing
P21-10G-5ug $ 215.00
P21-10G-10ug $ 310.00

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assay and reagents for drug discovery in lipid signaling pathways