PI4-Kinase beta (PI4Kb), active
Catalog No.: P32-10G
Description: Description: Full-length recombinant human PI4KB was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Enzyme obtained from SignalChem.
Molecular weight: ~120 kDa
Sequence: Full length
Tag: GST tag
Purity: >90 % (see TDS for lot specific information)
Specific Activity: >30 nmol/min/mg (see TDS for lot specific information)
Expression System: Sf9 insect cells using baculovirus
Genebank Number: BC000029
Applications: PI 4-Kinase assay , western blot.
Formulation: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Storage and stability: Store product at -70ºC. Stable up to one year.
Scientific Background: PI4KB is a member of the phosphatidylinositol 4-kinase family (PI4K) and is the first committed step in the production of the second messenger inositol-1,4,5-trisphosphate (PIP3). PI4KB regulates the golgi disintegration/reorganization during mitosis possibly via the phosphorylation of phosphatidylinositol. PI4KB is a type III enzyme that is sensitive to wortmannin and is responsible for regulating the synthesis of agonist-sensitive pools of polyphosphoinositides. PI4KB is critical for glucose-induced insulin secretion due to its capacity to regulate the release of secretory granules. PI4KB inhibits the insulin-stimulated translocation of glucose transporter-4 in mouse adipocytes through its interaction with neuronal calcium sensor-1.
PI(4)P Mass ELISA (K-4000E)
PI 4-Kinase Activity ELISA (K-4000K)
PIk-93; PI 4-kinase Inhibitor (B-0306)
assay and reagents for drug discovery in lipid signaling pathways