FS-3 (LysoPLD / Autotaxin substrate)

Catalog No.:  L-2000
MW: 1286.4
CAS: 889132-42-7

Description:  Echelon's fluorogenic Autotaxin (ATX) substrate FS-3 (L-2000) is a doubly labeled analog of LPC wherin the fluorophore is quenched through intramolecular energy transfer. Hydrolysis of the substrate produces an increase in fluorescence.

Autotaxin which has lysophospholipase D (lysoPLD) activity, cleaves choline from lysophosphatidylcholine forming lysophosphatidic acid (LPA), a potent mitogen that as been implicated in the pathophysiology of ovarian cancer. LysoPLD/ATX has been demonstrated to increase cell motility, neovascularization, proliferation, and aggressiveness of tumors and is upregulated in numerous cancer lineages (non-small cell lung , glioma, mammary carcinoma, renal cell carcinoma, hepatocellular carcinoma). In addition, dysregulation of the ATX/LPA pathway is central to the pathophysiology of idiopathic pulmonary fibrosis, rheumatoid arthritis, and other inflammatory diseases. Modulation of ATX activity through small-molecules is a currently underexplored target, but one with high potential for novel cancer and anti-inflammatory therapeutics.


-20 °C


5 mg / mL in H2O or MeOH

Featured in Publications:

1) Ferguson, C. G., C. S. Bigman, et al. (2006). "Fluorogenic phospholipid substrate to detect lysophospholipase D/autotaxin activity." Org Lett 8(10): 2023-6.
2) Baker, D. L., Y. Fujiwara, et al. (2006). "Carba analogs of cyclic phosphatidic acid are selective inhibitors of autotaxin and cancer cell invasion and metastasis." J Biol Chem 281(32): 22786-93.
3) Parrill, A. L., U. Echols, et al. (2008). "Virtual screening approaches for the identification of non-lipid autotaxin inhibitors." Bioorg Med Chem 16(4): 1784-95.
4) Hoeglund, A. B., H. E. Bostic, et al. (2010). "Optimization of a pipemidic acid autotaxin inhibitor." J Med Chem 53(3): 1056-66.
5) Tager, A.M. (2012) "Autotaxin Emerges as a Therapeutic Target for Idiopathic Pulmonary Fibrosis: Limiting Fibrosis by Limiting Lysophosphatidic Acid Synthesis" Am. J. Resp. Cell Mol. Biol. 47(5): 563-565.

Related Products:

Autotaxin Inhibitor Screening Kits: 96-well (K-4200) and 384-well (K-4200HTS)
Autotaxin Activity Assay (K-4100)
Autotaxin ELISA (K-5600)
ATX-Red AR-2, ATX Imaging Agent (L-2010)
Autotaxin Inhibitors: BrP-LPA
(L-7416), HA130 (B-0701), S32826 (L-3282), and Thio-ccPA (L-7118)
Autotaxin Enzyme, active (E-4000)

Notice to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patents 7,459,285 & 7,989,663. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email

Bulk discounts available, please email for information.

Options/Sizes Pricing
L-2000-100ug $ 284.00
L-2000-200ug $ 522.00
L-2000-1mg $ 2,294.00
Additional Information:
Tech Data Sheet - TDS_L-2000_Rev 4

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assay and reagents for drug discovery in lipid signaling pathways