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PI(3)P PIP Beads™


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Catalog No.:  P-B003a

Description:  PI(3)P PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(3)P per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.

Applications

  • PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize lipid binding proteins. Possible applications include the isolation of lipid-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.

Storage

  • 4 °C - do not freeze

Featured in Publications

1) Mosesson, Y., D. Chetrit, et al. (2009). "Monoubiquitinylation regulates endosomal localization of Lst2, a negative regulator of EGF receptor signaling." Dev Cell 16(5): 687-98.
2) Zhang, P., Y. Wang, et al. (2010). "Proteomic identification of phosphatidylinositol (3,4,5) triphosphate-binding proteins in Dictyostelium discoideum." Proceedings of the National Academy of Sciences 107(26): 11829-11834.

Being bio-reactive blog: "Lipid-Ligand interaction tools: lipids are not scary intermediates anymore"


Options/Sizes Pricing
P-B003a-0.1ml $ 99.00
P-B003a-1ml $ 515.00
Additional Information:
Tech Data Sheet - TDS_P-Bxxx_ Rev 7

Protocol - Protocol


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assay and reagents for drug discovery in lipid signaling pathways