Products

PI(4,5)P2 PIP Beads™


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Catalog No.:  P-B045a

Description:  PI(4,5)P2 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(4,5)P2 per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.

Storage

  • 4 °C - do not freeze

Applications

  • PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.

Featured in References

1) Gupta, S., J. C. Fanzo, et al. (2003). "T cell receptor engagement leads to the recruitment of IBP, a novel guanine nucleotide exchange factor, to the immunological synapse." J Biol Chem 278(44): 43541-9.
2) Kawahara, T. and J. D. Lambeth (2008). "Phosphatidylinositol (4,5)-bisphosphate modulates Nox5 localization via an N-terminal polybasic region." Mol Biol Cell 19(10): 4020-31.
3) Kim, S. K., H. Kim, et al. (2011). "Phosphatidylinositol phosphates directly bind to neurofilament light chain (NF-L) for the regulation of NF-L self assembly." Exp Mol Med 43(3): 153-60.

Being bio-reactive blog: "Lipid-Ligand interaction tools: lipids are not scary intermediates anymore"


Options/Sizes Pricing
P-B045a-100ul $ 99.00
P-B045a-1ml $ 515.00
Additional Information:
Tech Data Sheet - TDS_P-Bxxx_ Rev 7

Protocol - Protocol


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assay and reagents for drug discovery in lipid signaling pathways