Membrane Lipid Strips
assay and reagents for drug discovery in lipid signaling pathways
Catalog No.: P-6002
Description: Membrane Lipid Strips are 2 x 6 cm hydrophobic membranes that have been spotted with 100 pmol of fifteen different biologically important lipids found in cell membranes. They are used to determine how your protein of interest interacts with one or more of these lipids in a simple protein-lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.
Membrane Lipid Strips contain the following lipids at 100 pmol per spot: (click picture above for exact layout)Triglyceride
Phosphatidylinositol (4)-phosphate (PtdIns(4)P)
Phosphatidylinositol (4,5)-bisphosphate, (PtdIns(4,5)P2)
Phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3)
Phosphatidic acid (PA)
Each 10-pack of Membrane Lipid Strips includes a free vial of the PI(4,5)P2 specific binding protein (G-4501) for use as a positivie control. For a list other phosphoinositide binding proteins, click here.
Featured in Publications1) Sankarshanan, M., Z. Ma, et al. (2007). "Identification of a novel lipid raft-targeting motif in SRC homology 2-containing phosphatase 1." J Immunol 179(1): 483-90.
2) Lu, B. and C. Benning (2009). "A 25-amino acid sequence of the Arabidopsis TGD2 protein is sufficient for specific binding of phosphatidic acid." J Biol Chem 284(26): 17420-7.
3) Landeta, O., A. Landajuela, et al. (2011). "Reconstitution of proapoptotic BAK function in liposomes reveals a dual role for mitochondrial lipids in the BAK-driven membrane permeabilization process." J Biol Chem 286(10): 8213-30.
Being bio-reactive blog: "Lipid-Ligand interaction tools: lipids are not scary intermediates anymore"
Tech Data Sheet - TDS_ P-6002_rev 4
FAQ - Frequently Asked Questions
Protocol - PROTOCOL_strips_Arrays_v9