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Akt2, active


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Catalog No.:  E-K002

Description:  Recombinant full-length human Akt2 containing N-terminal His tag was expressed by baculovirus in Sf 9 insect cells.
The gene accession number is NM_001626.
This material is sold for research purposes only.
Specific Activity:
50 nmol phosphate incorporated into Akt substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32.

Scientific Background

  • Akt2 or Protein Kinase B alpha (PKBalpha) is a serine/threonine kinase that is a member of the Akt family. Akt2 like the other Akt members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2. Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (3).

Storage

  • Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.

References

  • 1. Coffer PJ, Jin J, Woodgett JR. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 ( Pt 1):1-13. 2. Anderson KE, Coadwell J, Stephens LR, Hawkins PT. Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91. 3. Sen P, Mukherjee S, Ray D, Raha S. Involvement of the Akt/PKB signaling pathway with disease processes. Mol Cell Biochem. 2003 Nov; 253(1-2): 241-6.


Options/Sizes Pricing
E-K002-5ug $ 263.00
E-K002-20ug $ 579.00
Additional Information:
Tech Data Sheet - E-K002


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assay and reagents for drug discovery in lipid signaling pathways