Products

PKA cb, active


Zoom
 
Catalog No.:  E-K033

Description:  Recombinant full-length human PKA cb containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells. The gene accession number is NM_002731.
This material is sold for research purposes only.
Specific Activity
29 umol phosphate incorporated into CREBtide substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Scientific Background

  • Most of the effects of cAMP are mediated through the phosphorylation of target proteins on serine or threonine residues by the cAMP-dependent protein kinase (AMPK). The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. The mammalian catalytic subunit has been shown to consist of three PKA gene products: C-alpha, C-beta, and C-gamma. Two PKA isoforms exist, designated types I and II, which differ in their dimeric regulatory subunits, designated RI and RII, respectively. Furthermore, there are at least four different regulatory subunits: RI-alpha, RI-beta, RII-alpha, and RII-beta. The cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The catalytic subunit C-beta of PKA (PKAcb) is a member of the Ser/Thr protein kinase family and is a catalytic subunit C-beta of AMPK. Berube et al. assigned the PKAcb to human chromosome 1 by Southern blot analysis of somatic c

Storage

  • Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.

References

  • 1. Berube, D.; Simard, J.; Sandberg, M.; Grzeschik, K.-H.; Gagne, R.; Hansson, V.; Jahnsen, T.: Assignment of the gene encoding the catalytic subunit C(beta) of cAMP-dependent protein kinase to the p36 band on chromosome 1. (Abstract) Cytogenet. Cell Genet. 58: 1850 only, 1991. 2. Simard, J.; Berube, D.; Sandberg, M.; Grzeschik, K.-H.; Gagne, R.; Hansson, V.; Jahnsen, T.: Assignment of the gene encoding the catalytic subunit C-beta of cAMP-dependent protein kinase to the p36 band on chromosome 1. Hum. Genet. 88: 653-657, 1992.


Options/Sizes Pricing
E-K033-5ug $ 147.00
E-K033-20ug $ 442.00
Additional Information:
Tech Data Sheet - E-K033


Add a specific quantity to your cart    *  


Back to the webstore startpage      Back to the product overview      Your Shopping Cart      Terms of Service


assay and reagents for drug discovery in lipid signaling pathways