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Catalog No.:  E-K051

Description:  Recombinant full length Rous sarcoma virus Src protein containing N-terminal GST tag was expressed by baculovirus in Sf9 insect cells.
The gene accession number is M11753.
This material is sold for research purposes only.
Specific Activity
116 nmol phosphate incorporated into Src substrate peptide per minute per mg proteins at 300C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay in 25ul reaction volume).

Scientific Background

  • Src family belongs to non-receptor tyrosine kinases. Src was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates. Src is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases. In addition to increasing cell proliferation, a key role of Src in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumour progression. Oncogenic forms of the Src alter cell structure, in particular the actin cytoskeleton and the adhesion networks that control cell migration, and also transmit signals that regulate proliferation and cell survival. Recent work indicates that Src plays a role in these functions by influencing the RhoA-ROCK pathway that controls contractile actin filament assembly, the STAT family of transcription factors needed for transformation, and the Cbl ubiquiti

Storage

  • Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoid repeated thawing and freezing.


Options/Sizes Pricing
E-K051-5ug $ 246.00
E-K051-20ug $ 541.00
Additional Information:
Tech Data Sheet - E-K051


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