Acid Sphingomyelinase Activity Assay Kit

Catalog No.:  K-3200

Description:  Echelon's Acid Sphingomyelinase Assay Kit is an enzyme assay that measures acid sphingomyelinase activity in biological samples through the direct hydrolysis of a fluorogenic substrate.

Assay sensitivity: 3.125 pmol/hour
Sample Type: tissue homogenates, cell lysates
Sample Volume: 20 uL/sample (for duplicate points)

Product Background: Sphingomyelinases catalyzes the hydrolysis of sphingomyelin into ceramide and phosphoryl choline; and is involved in programmed cell death (apoptosis), cell differentiation and cell proliferation. Sphingomyelinases are classified into five categories: acid sphingomyelinase (aSMase), secretory sphingomyelinase (sSMase), neutral Mg 2 +/- dependent sphingomyelinases (nSMase), neutral Mg 2 +/- independent sphingomyelinases and alkaline sphingomyelinase. Acid sphingomyelinase was the first described and best characterized of the sphingomyelinases. A deficiency of lysosomal acid sphingomyelinase leads to rapid neurodegeneration and death due to excessive accumulation of sphingomyelin (Niemann-Pick disease).
The Echelon Acid Sphingomyelinase Assay Kit uses a fluorogenic substrate specific for acid sphingomyelinase to provide a sensitive and homogenous method to measure the activity of aSMase in vitro. The kit provides all necessary reagents to measure the acid sphingomyelinase activity of 40 samples ran in duplicate.

Featured in Publication:
1. Li, J., W. Yu, et al. (2010). "alpha-TEA-induced death receptor dependent apoptosis involves activation of acid sphingomyelinase and elevated ceramide-enriched cell surface membranes." Cancer Cell Int 10: 40

Related Products:
Sphingomyelinase Activity Assay Kit (K-1800)
Neutral Sphingomyelinase Assay Service (T-1800)
Biotin Sphingomyelin (S-400B)

Options/Sizes Pricing
K-3200-1ea $ 467.00
Additional Information:
Tech Data Sheet - TDS_K-3200_Rev8

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assay and reagents for drug discovery in lipid signaling pathways