Sphingosine Kinase Activity Assay

Catalog No.:  K-3500

Description:  Echelon's Sphingosine Kinase Activity Assay is a luminescent assay that measures sphingosine kinase activity by detecting the decrease of ATP.

Sample Type: purified enzyme, cell lysate/homogenate
Sample Volume: 10 μL / sample (duplicate points)
Assay Incubation Time: 30 minutes to 2 hours

Product Background: Echelon's Sphingosine Kinase Activity Assay is prepared by the addition of sphingosine kinase, sphingosine (substrate) in reaction buffer and initiated by the addition of ATP. The reaction is then stopped by adding the ATP detector after the chosen reaction time. The luminescent signal is inversely correlated with the kinase activity. Echelon's sphingosine kinase activity assay is tested with Echelon's SPHK1 (E-K068) and also works with SPHK2 (E-K069)
Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). SPHKs have been implicated in proliferation, survival, migration and regulation of Ca2+ homoeostasis, development and regulation of the cardiovascular and nervous systems, inflammation, immunity, and cancer growth. There are two currently known SPHK isoforms, SPHK1 and SPHK2. It is believed that SPHK1 and SPHK2 have opposite functions, promoting cell growth and apoptosis respectively.

Featured in Publications:
1. Li, QF., Zhu, HY. et al. “Prokineticin-1/endocrine gland-derived vascular endothelial growth factor is a survival factor for human multiple myeloma cells.” Leuk Lymphoma 51:10
2. Cho, SY., Lee, HJ. et al. “Sphingosine kinase 1 pathway is involved in melatonin-induced HIF-1 inactivation in hypoxic PC-3 prostate cancer cells.” J Pineal Res 51:1
3.Guan, H., Song, L. et al. “Sphingosine kinase 1 regulates the Akt/FOXO3a/Bim pathway and contributes to apoptosis resistance in glioma cells.” PLoS One 6: 5

Related Products:
Sphingosine Kinase Enzyme: SPHK1, active and SPHK2, active
Sphingosine Kinase Inhibitor Screen (K-4400)
Sphingosine Kinase Inhibitor (B-0024)
Sphingosine: Sphingosine, Sphingosine-biotin, Sphingosine-fluorescein, Sphingosine-tetramethylrhodamine
Sphingosine 1 Phosphate ELISA: Kit (K-1900), Assay Service (T-1900)

Product Keywords: Sphingosine kinase, SPHK, SK, Sphingosine kinase-1, SPHK1 enzyme, SK1, Sphingosine kinase type 2, SPHK2, SK2

Options/Sizes Pricing
K-3500-1ea $ 454.00
Additional Information:
Tech Data Sheet - TDS_K-3500_Rev6

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assay and reagents for drug discovery in lipid signaling pathways