Autotaxin Activity Assay
Catalog No.: K-4100
Description: Echelon's Autotaxin Activity Assay gives a quantitative measurement of autotaxin (ATX) activity in biological samples by measuring changes in fluorescence over time.
Sample Volume: 400 μL / sample (50 μL per well, 8 wells per sample)
Sample Number: 10 samples (in duplicate) / 96 well assay
Sample Type: Currently this kit has been tested for the quantification of autotaxin activity in serum, plasma, ovarian ascites, and cell culture media.
Product Background: The assay developed by Echelon Biosciences Inc. uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence. Once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence.
Featured in Publications:1) Shelton, E. L., C. L. Galindo, et al. (2013). "Autotaxin Signaling Governs Phenotypic Heterogeneity in Visceral and Parietal Mesothelia." PLoS ONE 8(7): e69712.
2) Gaetano, C. G., N. Samadi, et al. (2009). "Inhibition of autotaxin production or activity blocks lysophosphatidylcholine-induced migration of human breast cancer and melanoma cells." Molecular Carcinogenesis 48(9): 801.
3) Braeuer, R. R., M. Zigler, et al. (2012). "Galectin-3 Contributes to Melanoma Growth and Metastasis via Regulation of NFAT1 and Autotaxin." Cancer Research 72(22): 5757-5766.
Autotaxin Inhibitor Screening Kits: 96-well (K-4200) and 384-well (K-4200HTS)
Autotaxin Substrate: FS-3 (L-2000)
Autotaxin Inhibitors: BrP-LPA (L-7416), HA130 (B-0701), and Thio-ccPA (L-7118 )
Autotaxin Enzyme, active (E-4000)
Notice to Purchaser:Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patent 7,989,663. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email firstname.lastname@example.org
Product Keywords: ATX, LPA, FS-3, LPC, lysophospholipase D
Tech Data Sheet - TDS _K-4100_ rev7