Product Background: The Autotaxin Inhibitor Assay, developed by Echelon Biosciences Inc., uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence. Once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence.
Percent autotaxin inhibition is determined by referencing no inhibitor controls. The autotaxin inhibitor, BrP-LPA, is supplied to positively control for autotaxin inhibition. The assay is resistant to modest concentrations of DMSO and is performed in a continuous assay format, which has the advantage of being less susceptible to error arising from mis-timing the addition of components to different sample wells.
Featured in Publications:
1. Altman, M.K., Gopal, V., Jia, W., Yu, S., Hall, H., Mills, G.B., McGinnis, A.C., Bartlett, M.G., Jiang, G., Madan, D., Prestwich, G.D., Xu, Y., Davies, M.A., & Murph, M.M., Targeting melanoma growth and viability reveals dualistic functionality of the phosphonothionate analogue of carba cyclic phosphatidic acid. 2010. Mol Cancer 9, 140.
2. Jiang, G., Madan, D., & Prestwich, G.D., Aromatic phosphonates inhibit the lysophospholipase D activity of autotaxin. Bioorg Med Chem Lett.
Autotaxin Assay kits: Autotaxin Activity Assay (K-4100), High Throughput Autotaxin Inhibitor Screening Kit (K-4200HTS)
Autotaxin Substrate; FS-3 (L-2000)
Autotaxin Inhibitors; BrP-LPA (L-7416), HA130 (B-0701), PF8380 (B-0702), and Thio-ccPA (L-7118 )
Autotaxin Enzyme, active (E-4000)
Notice to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patent 7,989,663. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email firstname.lastname@example.org
Product Keywords: ATX, LPA, FS-3, LPC, lysophospholipase D, BrP-LPA