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Sphingosine-1-Phosphate Lyase Overexpression Lysate, active


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Catalog No.:  E-5000L

Description:  Sphingsoine-1-phosphate lyase (SPL) (E.C.4.1.2.27) works as a key regulator of the final step of the sphingolipid degradative pathway by irreversibly breaking down the sphingosine-1-phosphate (S1P) to ethanolamine phosphate and hexadecenal. S1P signaling through its five receptors involves in many biological events such as proliferation, inflammation and apoptosis in cancer and other degenerative diseases.

HEK293 cell lysate with overexpressed SPL-GFP (green fluorescent protein).

Storage

-80°C. The enzyme is stable for 6 months from the date of receipt. Avoid repeat freeze thaw cycles.

References

1. Billich, A., et al. (2013). "Partial deficiency of sphingosine-1-phosphate lyase confers protection in experimental autoimmune encephalomyelitis." PLoS One 8(3): e59630.
2. Engel, N., et al. (2012). "Metabolic profiling reveals sphingosine-1-phosphate kinase 2 and lyase as key targets of (phyto-) estrogen action in the breast cancer cell line MCF-7 and not in MCF-12A." PLoS One 7(10): e47833.
3. Bedia, C., et al. (2009). "Synthesis of a fluorogenic analogue of sphingosine-1-phosphate and its use to determine sphingosine-1-phosphate lyase activity." Chembiochem 10(5): 820-2.
4. Reiss,U., et al. (2004) "Sphingosine-phosphate Lyase Enhances Stress-induced Ceramide Generation and Apoptosis." J. Biol. Chem. 279(2): 1281-1290.

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Options/Sizes Pricing
E-5000L-250ug $ 250.00
Additional Information:
Tech Data Sheet - TDS_E-5000L_Rev1


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assay and reagents for drug discovery in lipid signaling pathways