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Anti-PEth Autologous IgG ELISA


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Catalog No.:  K-5000A

Description:  Echelon Bioscience’s Human Anti-PEth Autologous IgG ELISA is a direct assay in which the signal is proportional to the amount of autologous anti-PEth IgG contained in the sample. The serum (or plasma) sample is added to a PEth-coated plate for binding. A peroxidase-linked secondary detector and colorimetric substrate are used to detect the amount of anti-PEth antibody bound to the plate. The colorimetric signal is read at absorbance 450 nm and is directly proportional to the amount of autologous anti-PEth IgG in the sample.

Ethanol (EtOH) has multiple effects on humans and other animals. Alcohol addiction and organ damage are active areas of research with a need for research reagents and tools.1 Phosphatidylethanol (PEth) is a direct metabolite of EtOH and has been described as a long-lived biomarker for alcohol ingestion.2, 3 It is possible that PEth is involved in the neurological4, protective5, and harmful6 effects of alcohol, but the mechanisms are still being explored.
Autologous anti-PEth antibodies are found in lower abundance in serum from heavier drinkers. This inverse relationship between anti-PEth IgG levels and alcohol ingestion was first reported, by Nissinen et. al.7 Evaluation of serum samples of varying consumption habits has demonstrated a high degree of correlation with ingested alcohol. Measuring these autologous anti-PEth IgG levels may serve as an effective biomarker in monitoring alcohol use.

Storage

4 °C

References

1. Freeman, W. M.; Vrana, K. E. Future prospects for biomarkers of alcohol consumption and alcohol-induced disorders. Alcohol Clin Exp Res 2010, 34, 946-54.
2. Viel, G.; et al. Phosphatidylethanol in blood as a marker of chronic alcohol use: a systematic review and meta-analysis. Int J Mol Sci 2012, 13, 14788-812.
3. Wurst, F. M.; et al. Phosphatidylethanol: normalization during detoxification, gender aspects and correlation with other biomarkers and self-reports. Addict Biol 2010, 15, 88-95.
4. Esteban-Pretel, G.; et al. Polyphosphoinositide metabolism and Golgi complex morphology in hippocampal neurons in primary culture is altered by chronic ethanol exposure. Alcohol Alcohol 2012, 48, 15-27.
5. Foster, D. A.; et al. Reduced mortality and moderate alcohol consumption: the phospholipase D-mTOR connection. Cell Cycle 2010, 9, 1291-4.
6. Pannequin, J.; at al. Phosphatidylethanol accumulation promotes intestinal hyperplasia by inducing ZONAB-mediated cell density increase in response to chronic ethanol exposure. Mol Cancer Res 2007, 5, 1147-57.
7. Nissinen, A.E.; et al. Low plasma antibodies specific for phosphatidylethanol in alcohol abusers and patients with alcoholic pancreatitis. Addict Biol, 2011. 17(6): p. 1057-67.

Related Products
Phosphatidylethanol ELISA (K-5000)
Anti-Phosphatidylethanol Antibody (Z-PETH)
Phosphatidylethanol coated beads (P-BPEth)
Synthetic Phosphatidylethanols


Options/Sizes Pricing
K-5000A-1kit $ 595.00
Additional Information:
Tech Data Sheet - K-5000A


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