Dibromobimane is a thiol reactive protein cross linking reagent that generates a fluorescent product when both of the alkylating groups have reacted with proteins. Dibromobimane has an excitation/ emission spectra of 390/450 nm.
References
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4) Lindzen, M., K.-E. Gottschalk, et al. (2006). “Structural Interactions between FXYD Proteins and Na+,K+-ATPase: α/β/FXYD SUBUNIT STOICHIOMETRY AND CROSS-LINKING.” Journal of Biological Chemistry 281(9): 5947-5955.
