The PI(3,4)P2 Mass ELISA measures the amount of PI(3,4)P2 extracted from cells by means of a competitive ELISA (96 well format).

Assay Range: 300 to 1.23 pmol PI(3,4)P2
Sample Type: Dried down lipid extracted from cells
Sample Volume: 120 µL/sample, run in duplicate.

The PI(3,4)P2 Mass ELISA is a competitive ELISA where the signal is inversely proportional to the amount of PI(3,4)P₂ produced. This allows the user to measure the amount of PI(3,4)P₂ extracted from cells by means of standard 96 well ELISA format. Eliminating the need for radioactivity and thin layer chromatography.

Once PI(3,4)P₂ has been extracted from cellular samples, it is incubated with a PI(3,4)P₂ detector, then added to the PI(3,4)P₂-coated plate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric substrate is used to detect PI(3,4)P₂ detector binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(3,4)P₂ extracted from cells.

Keywords: PI(3,4)P2, PI34P2, PtdIns(3,4)P2

Categories	Kits & Assays
Filter	ELISA, Lipid Detection, Phosphoinositides, PI(3,4)P2
Shipping Temp	Gel Ice
Storage	4-8 °C

Technical Data Sheet

PIP Mass FAQ

1) Mondal, S., et al. (2012). “Phosphoinositide lipid phosphatase SHIP1 and PTEN coordinate to regulate cell migration and adhesion.” Molecular Biology of the Cell. 23, 1219-1230, DOI:10.1091/mbc.E11-10-0889
2) Guo, S. T., et al. (2015). “INPP4B is an oncogenic regulator in human colon cancer.” Oncogene. 35, 3049–3061, DOI:10.1038/onc.2015.361
3) Reed, D. E. and K. M. Shokat (2017). “INPP4B and PTEN Loss Leads to PI-3,4-P2 Accumulation and Inhibition of PI3K in TNBC.” Mol Cancer Res 15(6): 765-775. DOI:10.1158/1541-7786.MCR-16-0183
4) Leitner, M. G., et al. (2018). “A126 in the active site and TI167/168 in the TI loop are essential determinants of the substrate specificity of PTEN.” Cellular and Molecular Life Sciences 75(22): 4235-4250.DOI:10.1007/s00018-018-2867-z
5) Hawse, W. F. and R. T. Cattley (2019). “T cells transduce T-cell receptor signal strength by generating different phosphatidylinositols.” J Biol Chem 294(13): 4793-4805. DOI:10.1074/jbc.RA118.006524

PI(3,4)P2 Mass ELISA

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PI(3,4)P2 Mass ELISA

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