Protein Tyrosine Phosphatase Substrate, fluorogenic

Molecular Weight: 1440. [Read More...]

Description
Additional Information

Molecular Weight: 1440.49
Salt Form: Acetate
Purity: >96%
Sequence (3-letter): MCA-Gly-Asp-Ala-Glu-pTyr-Ala-Ala-Lys(DNP)-Arg-NH2
Sequence (1-letter): MCA-GDAE(pTyr)AA(Lys(DNP))R-NH2
Storage: -20 °C or below

MCA-Gly-Asp-Ala-Glu-pTyr-Ala-Ala-Lys(DNP)-Arg-NH2 acts as a substrate for many protein tyrosine phosphatases. After dephosphorylation, the substrate is treated with chymotrypsin which cleaves the Tyr-Ala bond resulting in an increase in fluorescence (Ex 325 nm, Em at 393 nm).

References
Biochem. J. (1999), 343, 385-391