Lysophosphatidic Acid (LPA) Screening Service

The Lysophosphatidic Acid (LPA) Screening Service uses the LPA ELISA II (cat# K-2800s) to quantify LPA in biological samples. LPA is a serum-derived phospholipid involved in diverse cellular processes such as cell proliferation, chemotaxis, platelet aggregation, wound healing, angiogenesis, tumor invasion, and smooth muscle contraction. Recent research indicates LPA may play a significant role in the pathophysiology of cancer and may be used as a biomarker for ovarian cancer. Several literature citations indicate plasma LPA levels are elevated in ovarian cancer patients.

• Echelon has 25 years of ELISA expertise and 15 years experience in the LPA field.
• Your samples are cataloged and stored according to your requirements.
• The LPA Screening Service will run each sample in duplicate.
• Results are provided as an average of the duplicate points in μM LPA.
• Usually, results are provided 2 weeks from receipt of samples. We can accommodate tighter schedules if needed.

Contact us for a quote on your custom assay service.

Description

Assay Details

LPA is quantified using the LPA ELISA II (cat # K-2800s). The LPA ELISA II is a sensitive and robust method for quantification of LPA in a competitive ELISA format.  The concentration of LPA in the sample is determined using a standard curve of known amounts of C18:3 LPA.

Assay Range: 0.064 μM to 1,000 μM LPA
Inter-assay CV: 8.9-15.33%
Intra-assay CV: 6.5-8.7%

Sample Considerations

The LPA Screening Service has been tested for the quantification of LPA in serum and plasma.  Serum and plasma show similar basal levels of LPA. Some plasma samples (those with EDTA) may interfere with the assay. 

Sample Type: Serum and Plasma (without EDTA)

Sample Volume: minimum of 30 μL is required

Species of sample: This assay is not species specific. Mouse and human samples have been tested.

LPA species: (in order of high to low) C18:3 > C18:2 > “plasma” LPA > C20:4 > C14:0 > C18:1 > C16:0 > C18:0. The assay does not pick up LPA C20:0. The “plasma” LPA ratio was made according to the average of 2 literature references (Murph 2007 & Sutphen 2004) and contained C16:0 : C18:0 : C18:1 : C18:2 : C20:4 = 2.8:1.0:1.5:2.6:3.0

Storage and shipment of samples:

• Freeze all samples before shipping and ship on 3-5 lbs of dry ice, if possible.
• If samples cannot be frozen, use leakproof caps and vials.
• Label vials well. If vials leak, the solution can remove poor labeling.
• Please ensure all shipments containing human tissue or fluids are appropriately labeled as biohazard and packaged to prevent leakage during transport.
• Contact your service coordinator and let them know the samples are on their way.

Cost

The minimum sample number for this service is 20. Example pricing is shown below and custom sample numbers can be accommodated. 

20 SAMPLES     $2,702.00
30 SAMPLES     $3,071.00
40 SAMPLES     $4,053.00

Standard pricing is for each sample to be ran in duplicate wells. This means that 20 samples = 40 assay wells. If you need to run greater or fewer samples, contact us for pricing. If you want your samples ran in singlet or triplicate, let us know! We will adjust pricing.

Contact Us

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Publications

Citations listed are from the LPA ELISA II (catalog number K-2800S).

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