Echelon Biosciences is now the sole authorized distributor for Select-HA™ products from Hyalose, LLC.

Spring 2019 Press Release

Echelon now offers the follwing Hyalose products:

Select-HA™

Select-HA™ is a hyaluronic acid (HA) preparation of uniform and narrow size distribution prepared by in vitro synthesis using recombinant Pasteurella multocida hyaluronan synthase¹. Commercial HA is often purified from natural sources and therefore contains a mixture of various sizes HA (figure 1). 

The enzymatic quasi-monodisperse method used in Select-HA™ production allows tight control of the HA size. With low endotoxin levels (<0.1 EU/mg), Select-HA™ is even suitable for cell culture experiments.

Available sizes: 50 kDa, 500 kDa, 601 kDa, 1000 kDa

Biotinylated Select-HA™

Biotinylated Select-HA™ is the biotin version of the Select-HA™ which allows the researcher to immobilize or detect the Select-HA™ through the avidin-biotin interactions. A single biotin is conjugated at the reducing-end of each Select-HA™ molecule which results in a more homogeneous conjugate compared with biotinylation of the HA carboxyl groups which is variable in both loading and conjugate locations. In addition, the reducing-end conjugation provides advantage over the HA carboxyl group biotinylation by not masking the HA functional groups and allowing access to the entire HA polymers.

Available sizes: 50 kDa, 250 kDa, 500 kDa, 1000 kDa

Select-HA™ Ladder

Select-HA™ Ladder contains five different molecular weights of Select-HA™ that can be used as size markers for agarose gel determination of HA sizes in samples. The Select-HA™ LoLadder contains HA markers range from 30 kDa to 500 kDa while the Select-HA™ HiLadder contains HA markers range from 500 kDa to 1500 kDa. In addition, we also offer the Select-HA™ MegaLadder which covers the high molecular weight HA from 2 MDa to 8 MDa. Together, the Select-HA™ Ladder provides a quick and simple method to determine HA sizes using agarose electrophoresis.

nanoHA™

nanoHA™ is a HA oligomer prepared by chemoenzymatic synthesis with immobilized enzyme reactors². The reducing-end is N-acetylglucosamine. nanoHA™ allows researcher to study the minimal HA disaccharides units that are needed for its potential binding partners.

Available oligomers: 5-mer

References

1. Jing, W.; DeAngelis, P. L., Synchronized chemoenzymatic synthesis of monodisperse hyaluronan polymers. J Biol Chem 2004, 279 (40), 42345-9.
2. DeAngelis, P. L.; Oatman, L. C.; Gay, D. F., Rapid chemoenzymatic synthesis of monodisperse hyaluronan oligosaccharides with immobilized enzyme reactors. J Biol Chem 2003, 278 (37), 35199-203.
3. Jing, W.; Haller, F. M.; Almond, A.; DeAngelis, P. L., Defined megadalton hyaluronan polymer standards. Anal Biochem 2006, 355 (2), 183-8.