Echelon Biosciences is now the sole authorized distributor for Select-HA™ products from Hyalose, LLC.

Echelon now offers the follwing Hyalose products:

Select-HA™

Select-HA™ is a hyaluronic acid (HA) preparation of uniform and narrow size distribution prepared by in vitro synthesis using recombinant Pasteurella multocida hyaluronan synthase1. Commercial HA is often purified from natural sources and therefore contains a mixture of various sizes HA (figure 1). 

The enzymatic quasi-monodisperse method used in Select-HA™ production allows tight control of the HA size. With low endotoxin levels (<0.1 EU/mg), Select-HA™ is even suitable for cell culture experiments.

Available sizes: 50 kDa500 kDa601 kDa1000 kDa

Biotinylated Select-HA™

Biotinylated Select-HA™ is the biotin version of the Select-HA™ which allows the researcher to immobilize or detect the Select-HA™ through the avidin-biotin interactions. A single biotin is conjugated at the reducing-end of each Select-HA™ molecule which results in a more homogeneous conjugate compared with biotinylation of the HA carboxyl groups which is variable in both loading and conjugate locations. In addition, the reducing-end conjugation provides advantage over the HA carboxyl group biotinylation by not masking the HA functional groups and allowing access to the entire HA polymers.

Available sizes: 50 kDa250 kDa500 kDa1000 kDa

Select-HA™ Ladder

Select-HA™ Ladder contains five different molecular weights of Select-HA™ that can be used as size markers for agarose gel determination of HA sizes in samples. The Select-HA™ LoLadder contains HA markers range from 30 kDa to 500 kDa while the Select-HA™ HiLadder contains HA markers range from 500 kDa to 1500 kDa. In addition, we also offer the Select-HA™ MegaLadder which covers the high molecular weight HA from 2 MDa to 8 MDa. Together, the Select-HA™ Ladder provides a quick and simple method to determine HA sizes using agarose electrophoresis.

nanoHA™

nanoHA™ is a HA oligomer prepared by chemoenzymatic synthesis with immobilized enzyme reactors2. The reducing-end is N-acetylglucosamine. nanoHA™ allows researcher to study the minimal HA disaccharides units that are needed for its potential binding partners.

Available oligomers5-mer

References

  1. Jing, W.; DeAngelis, P. L., Synchronized chemoenzymatic synthesis of monodisperse hyaluronan polymers. J Biol Chem 2004, 279 (40), 42345-9.
  2. DeAngelis, P. L.; Oatman, L. C.; Gay, D. F., Rapid chemoenzymatic synthesis of monodisperse hyaluronan oligosaccharides with immobilized enzyme reactors. J Biol Chem 2003, 278 (37), 35199-203.
  3. Jing, W.; Haller, F. M.; Almond, A.; DeAngelis, P. L., Defined megadalton hyaluronan polymer standards. Anal Biochem 2006, 355 (2), 183-8.
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