PIP Arrays are 4 x 5 cm hydrophobic membranes that have been spotted with a concentration gradient of all eight phosphoinositides. They are used to determine the relative degree of binding of your protein of interest towards one or more of these lipids in a simple protein lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.
Each PIP Array has 100, 50, 25, 12.5, 6.25, 3.13, and 1.56 pmol of the following phosphoinositides:
Phosphatidylinositol (PtdIns)
Phosphatidylinositol (3)-phosphate (PtdIns(3)P)
Phosphatidylinositol (4)-phosphate (PtdIns(4)P)
Phosphatidylinositol (5)-phosphate (PtdIns(5)P)
Phosphatidylinositol (3,4)-bisphosphate (PtdIns(3,4)P2 )
Phosphatidylinositol (3,5)-bisphosphate (PtdIns(3,5)P2)
Phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P2)
Phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3)
Each 5-pack of PIP Arrays includes a free vial of the PI(4,5)P2 specific binding protein for use as a positive control. For a list other phosphoinositide binding proteins, click here.
Storage
Store at 2-8 °C. Product is moisture and light sensitive
Featured in Publications
1) Gozani, O., P. Karuman, et al. (2003). “The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor.” Cell 114(1): 99-111.
2) Lindsay, A. J. and M. W. McCaffrey (2004). “The C2 domains of the class I Rab11 family of interacting proteins target recycling vesicles to the plasma membrane.” J Cell Sci 117(19): 4365-4375.
3) Andersson, E., F. Schain, et al. (2006). “Interaction of human 15-lipoxygenase-1 with phosphatidylinositol bisphosphates results in increased enzyme activity.” Biochim Biophys Acta 1761(12): 1498-505.
4) Guittard, G., A. Gerard, et al. (2009). “Cutting Edge: Dok-1 and Dok-2 Adaptor Molecules Are Regulated by Phosphatidylinositol 5-Phosphate Production in T Cells.” J Immunol 182(7): 3974-3978.
Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”
