Dibromobimane is a thiol reactive protein cross linking reagent that generates a fluorescent product when both of the alkylating groups have reacted with proteins. Dibromobimane has an excitation/ emission spectra of 390/450 nm.
References
1) Mornet, D., K. Ue, et al. (1985). “Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.” Proceedings of the National Academy of Sciences 82(6): 1658-1662.
2) Konno, K., K. Ue, et al. (2000). “Consequences of placing an intramolecular crosslink in myosin S1.” Proceedings of the National Academy of Sciences 97(4): 1461-1466.
3) Chen, Z., B. L. Akin, et al. (2006). “Cross-linking of C-terminal Residues of Phospholamban to the Ca2+ Pump of Cardiac Sarcoplasmic Reticulum to Probe Spatial and Functional Interactions within the Transmembrane Domain.” Journal of Biological Chemistry 281(20): 14163-14172.
4) Lindzen, M., K.-E. Gottschalk, et al. (2006). “Structural Interactions between FXYD Proteins and Na+,K+-ATPase: α/β/FXYD SUBUNIT STOICHIOMETRY AND CROSS-LINKING.” Journal of Biological Chemistry 281(9): 5947-5955.
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