Membrane Lipid Strips are 2 x 6 cm hydrophobic membranes that have been spotted with 100 pmol of fifteen different biologically important lipids found in cell membranes. They are used to determine how your protein of interest interacts with one or more of these lipids in a simple protein-lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.
Membrane Lipid Strips contain the following lipids at 100 pmol per spot:
Phosphatidylinositol (4)-phosphate (PtdIns(4)P)
Phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P2)
Phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3)
Phosphatidic acid (PA)
Storage: Store at 2-8 °C. Product is moisture and light sensitive
1) Sankarshanan, M., Z. Ma, et al. (2007). “Identification of a novel lipid raft-targeting motif in SRC homology 2-containing phosphatase 1.” J Immunol 179(1): 483-90.
2) Lu, B. and C. Benning (2009). “A 25-amino acid sequence of the Arabidopsis TGD2 protein is sufficient for specific binding of phosphatidic acid.” J Biol Chem 284(26): 17420-7.
3) Landeta, O., A. Landajuela, et al. (2011). “Reconstitution of proapoptotic BAK function in liposomes reveals a dual role for mitochondrial lipids in the BAK-driven membrane permeabilization process.” J Biol Chem 286(10): 8213-30.
Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”