Full-length recombinant human PI4KIIB was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Enzyme obtained from SignalChem.
Molecular weight: ~84 kDa
Sequence: Full length
Species: Human
Tag: GST tag
Purity: >90 % (see TDS for lot specific information)
Specific Activity: >30 nmol/min/mg (see TDS for lot specific information)
Expression System: Sf9 insect cells using baculovirus
Genebank Number: NM_018323
Applications: PI 4-Kinase assay , western blot.
Formulation: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Storage and stability: Store product at -80ºC. Stable up to one year
Product Description:
PI4KIIB is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate which are immediate precursor of several important signaling and scaffolding molecules. PI4KIIB is primarily a cytosolic PI4K that is recruited to membranes where it stimulates phosphatidylinositol 4,5-bisphosphate syntheses. PI4KIIB uses phosphatidylinositol as their primary substrate and has no activity on phosphatidylinositol monophosphates. PI4KIIB is a restricted minor histocompatibility antigen in patients that had been successfully treated with donor lymphocyte infusions for relapsed chronic myeloid leukemia after allogeneic stem cell transplantation.
