Protocols

Below are documents for our our most commonly requested protocols. For more specific questions not listed here or additional product troubleshooting, please see our FAQ page or contact our Technical Support.

Strips and Arrays

Echelon’s Lipid Strips and Arrays are simple, but highly effective and widely used assays for determining lipid-protein interactions. Here we provide an extensive protocol as a guide and reference for use with your protein of interest. The protocol includes suggestions for positive controls and buffers as well as general recommendations for a successful assay.

Strips and Arrays Protocol

Immunocytochemistry – Proteins

Immunocytochemistry (ICC) is a universally accepted technique for visualizing targets of interest in intact cells. While the technique is relatively straightforward, care must be taken when considering appropriate buffers, fixation conditions, and incubation times. Here we describe an optimized, general protocol for ICC of proteins in fixed cells.

Protein ICC Protocol

Immunocytochemistry – Lipids

Unlike immunocytochemistry (ICC) with proteins, ICC staining of lipids requires a great deal more planning and consideration. Lipids can reside in the membranes of multiple intracellular compartments, all of which will respond to permeabilization and fixation reagents differently. Here we outline optimized procedures for staining of the plasma membrane and Golgi apparatus with anti-lipid antibodies.

Lipid ICC Protocol

Lipid Beads

Lipid BeadsTM provide an ideal platform for the in vitro identification of lipid-protein interactions using either purified recombinant proteins or transfected cell lysates. Here we describe an optimized starting protocol for using Lipid BeadsTM in a protein pull-down experiment.

Lipid Coated Beads Protocol

Hyaluronic Acid – Size Determination

Gel electrophoresis provides the easiest way to estimate hyaluronic acid (HA) molecular weight from biological samples. However, choosing the correct running conditions is critical for a successful experiment. Here, we provide a general guideline for HA gel electrophoresis with a summary table of HA molecular weight in biological samples to facilitate your HA Gel Electrophoresis experiment.

HA Gel Electrophoresis Protocol

Liposome Preparation

This protocol is recommended as a general guide for preparation of liposomes from lyophilized lipids. Additional considerations and common troubleshooting points are addressed in our Liposome FAQ.

Liposomes Protocol  
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